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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Broadening the ARMC2 mutational phenotype: linking multiple morphological abnormalities of the Flagella to Pulmonary Manifestations in Primary Ciliary Dyskinesia

Fig. 3

Expression Analysis and Location of ARMC2 Protein in Sperm Flagella. A ARMC2 immunostaining in human spermatozoa from a normal control and men harboring ARMC2 variants. Sperm cells were stained with anti-ARMC2 (green) and anti-Ac-Tubulin (red) antibodies. DNA was counterstained with Hoechst as a nuclei marker. ARMC2 immunostaining is localized along the entire flagella but is almost absent in the sperm flagella of men harboring ARMC2 variants. Scale bar: 10 μm. B Immunoblotting analysis of ARMC2 protein in sperm lysates from a normal control and men harboring ARMC2 variants (P1 and P2). WB assays suggested that the level of ARMC2 was reduced significantly in the sperm from men harboring ARMC2 variants when compared to the level in sperm from a normal control man. β-Actin was used as a loading control

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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