Fig. 4
Proteome analysis of FSK stimulated BeWo cells cultured under normal (Ca2+ N) or under low calcium (Ca2+ L) conditions. (A) Heatmap of calcium dependent dysregulated proteins in FSK stimulated cells. (B) Increased signaling pathways in stimulated cells cultured under normal Ca2+ (left) and under low Ca2+ (right). Normalized abundance value of each sample was used for quantification. (C) Heatmap of all quantified proteins in stimulated BeWo cells. (D) Calcium dependent identification and quantification profile. (E) Volcano plot of quantified proteins in proteome analysis of stimulated BeWo cells. Identification of proteins that are upregulated by the activation of adenylyl cyclase (via FSK) in response to calcium (p < 0.05), N = 4, unpaired t-test. Blue point = Checked proteins from Fig. 4D, red point = upregulated in Ca2+ N FSK, green points = upregulated in Ca2+ L FSK, grey point = no significant different expression (F). Differentially expressed proteins in differentiated BeWo cells shown in 4 C are grouped according to their functional categories of gene ontology. Proteins in red are up-regulated in FSK stimulated cells cultured under high calcium