Fig. 3

Proteome analysis of RIPA lysates from BeWo cells cultured under normal Ca²⁺ condition (0.94mM = Ca²⁺ N) and 48 h incubation with DMSO (ctrl) or with FSK (2 biological and 2 technical replicates). (A) Heatmap of all dysregulated proteins (491) during differentiation of cytotrophoblasts to STB. Normalized abundance value of each sample was used for quantification. (B) Increased signaling pathways before (left) and after (right) induction of syncytialization with FSK in cells cultured under normal Ca²⁺ conditions. (C) Gene ontology analysis (ShinyGO) to determine increased signaling pathways of selected dysregulated proteins due to placental expression (UniProt). (D) Differentially expressed proteins shown in C are grouped according to their functional categories of gene ontology. (E) Venn diagram of identified and quantified proteins before (Ca²⁺ N ctrl) and after (Ca²⁺ N FSK) FSK induced syncytialization. (F) Volcano plot of quantified proteins in DMSO (Ca²⁺ N ctrl) - and FSK treated cells (Ca²⁺ N FSK). N = 4, unpaired t-test. Blue point = proteins of D, red point = upregulated in Ca²⁺ N FSK, green points = upregulated in Ca²⁺ L FSK, grey point = no significant different expression