Fig. 1

Relative mRNA expression levels of TAS2R14 (A), TAS2R43 (B), StAR (C), and CYP171A (D) in GCs (n = 30) were measured under various conditions: untreated (Ctrl), treated with BCA 10 µM for 24-h, and co-treated with BCA 10 µM plus either PRO 1 mM (BCA + PRO), SBA 30 µM (BCA + SBA), or CITRO 150 µM (BCA + CITRO) for 24-h. Antagonists were preincubated for 1 h before the addition of BCA. The means ± SD mRNA levels are expressed as a relative fold change compared to Ctrl after normalizing on the housekeeping gene GADPH. Statistical significance was calculated by Ordinary One-way ANOVA test (multiple comparisons) and indicated with letters. E-F Progesterone (E) and E2 (F) secretion from GCs, expressed as fold change relative to the control (set to 1). Different letters and * indicate statistically significant differences (p ≤ 0.05)