Fig. 3

Drosophila mutants model ovarian failure. (A) Representative images of DAPI-stained Drosophila ovaries show three to 4-day-old female flies with alterations in egg chamber morphology compared to control females (y, w). Drosophila lines are indicated on the left of the panel and the genes that were silenced by RNAi or that were knocked out by transposon insertions are indicated on the top. Fluorescent images were captured at 40x magnification. (B) Oocyte maturation rate in ak2 deficient and cdc27 mutant female ovaries. Data are expressed as percentages (%) over the respective controls (mean of minimum three replicates ± standard deviation). Arrows are included to show the structures in the pictures: eggs, oocytes, germ cells (GC), ovaries. *p < 0.05