Fig. 2

Gene expression with predicted deleterious mutations in Drosophila ovaries and their effects on female fertility. (A) Relative mRNA expression of prioritized OF-related genes in 3-day-old female flies with the corresponding gene silencing or knockout. Gene expression levels were normalized to tubulin and are expressed as fold-changes in expression compared to controls (mean of four replicates ± standard deviation). (B) Quantification of the viable offspring produced by female flies of each genotype. Data was expressed as percentages over wild-type controls (mean of 23 replicates ± standard deviation). *p < 0.05; **p < 0.01; ***p < 0.001