Skip to main content
Fig. 7 | Reproductive Biology and Endocrinology

Fig. 7

From: Analysis of single-cell RNA sequencing in human oocytes with diminished ovarian reserve uncovers mitochondrial dysregulation and translation deficiency

Fig. 7

Prediction of the potential transcriptional factors (TFs) of the hub genes. The KnockTF database (https://bio.liclab.net/KnockTFv2/) was used to predict the transcriptional factors associated with hub genes involved in mitochondrial function (A) and translation (B). The y-axis of the bar chart showed the predicted transcription factors, while the x-axis represented the number of hub genes regulated by each transcription factor. The color scheme indicated p-value. (C) Venn diagrams showed the intersection of TFs identified from (A) and (B). (D) The relative expression levels of five TFs were determined based on scRNA-seq. (E) The Venn network displayed the hub genes specifically targeted by PLAG1. (F) The violin plot displayed the expression of IGF2 gene in control and DOR oocytes based on scRNA-seq. **** for p-value < 0.0001. (G) Expression of PLAG1 and IGF2 was determined by RT-qPCR to validate the scRNA-seq data. Significance was indicated by * for p-value < 0.05

Back to article page

Reproductive Biology and Endocrinology

ISSN: 1477-7827

Contact us