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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Cordycepin improves hyperactivation and acrosome reaction through adenosine receptors during human sperm capacitation in vitro

Fig. 1

Cordycepin increased acrosome reaction and hyperactivation during human sperm capacitation. (A) Human sperm was stained using fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA). Sperm with uniform and bright green fluorescence in the head were considered to have acrosome integrity (AI). Sperm that only had weak fluorescence in the equatorial region, or even no fluorescence in the head were regarded as exhibiting an acrosome reaction (AR). (B) The number of AR sperm divided by the total number of sperm was calculated to yield the AR ratio. Dead sperm were stained using propidium iodide (PI) to calculate nonviable cell percentage (NVC%). Data are presented as means ± standard deviation (SD; n = 3). *P < 0.05 (compared with the dimethyl sulfoxide [DMSO] group). (C) The effects of different concentrations of cordycepin on sperm motility parameters. Data are presented as means ± SD (n = 5). *P < 0.05 (compared with the DMSO group)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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