Fig. 3

Establishment of the prediction model for contamination of IVF-PGTA. A Workflow of artificially mixing embryo/maternal or embryo/paternal DNA in different proportions for contamination analysis. B Schematic of the contamination origin based on maternal or paternal analysis. MC represents the LLR value for maternal bias, and PC represents the LLR value for paternal bias. C Establishment of the quantitative standard curve for contamination analysis. D-F LLR analysis for interpretation of parental contamination; the Z score was used to quantify the anomaly. NA: 5–10 biopsied trophectoderm cells without parental contamination; P20/M20, P30/M30, P50/M50: paternal or maternal gDNA/embryo DNA = 2:8, 3:7, 5:5, respectively. The above data are representative of three independent experiments, and the error bars indicate the means ± SEMs