Fig. 2

Validation of the effects of parental cells subjected to different single-cell WGA approaches. A We selected 1, 5, 10, 15, 20, and 25 individual mature sperm cells for tubing (black arrowheads); the WGA products of different numbers of sperm cells obtained with PicoPLEX (B) and with tenfold dilutions of MDA (C) were analysed by gel electrophoresis. PBS served as the negative control (NC); 5–10 biopsied TEs were used as the positive control; M: 15 kb DNA ladder. D Statistical analysis of the amplification yield of sperm cells with PicoPLEX and MDA. E We isolated 1, 2, and 3 single cumulus cells from the tubing (black arrowheads). F WGA products of 1, 2, and 3 cumulus cells with PicoPLEX and MDA were analysed by gel electrophoresis. The cycle values of all samples are shown in Supplementary Table 4. G Statistical analysis of the amplification yield of cumulus cells with PicoPLEX and MDA. CNV analysis of 25 sperm cells (H), one cryopreserved cumulus cell (I), and 10 cocultured cumulus cells (J). The scale bars represent 75 µm. M: 15 kb DNA marker. The above data are representative of three independent experiments, and the error bars indicate the means ± SEMs