Fig. 7
Nur77 activates mitophagy in the ovaries of reproductive aging mice. A The mRNA expression levels of LC3, p62, Parkin and PINK1 in each group after overexpression of Nur77. B and C The protein expression levels of LC3, p62, Parkin and PINK1 in each group after overexpression of Nur77. D Immunofluorescence staining was used to detect the localization and expression of LC3 AND TOM20 in the ovarian tissues of the each group after overexpression of Nur77. Scale: 50 μm. E Electron micrographs showed the normal mitochondrial morphology (green arrow), mitochondrial damage (red arrow), and mitophagy body formation (yellow arrow), in ovarian tissues. Scale: 2.0 μm, 500 nm. Error bars, mean ± SEM. n = 6–8 per group. *P < 0.05, **P < 0.01, ***P < 0.001