Fig. 2
The activity of mitophagy decreased and the level of apoptosis increased in the ovary of reproductively aging mice. A The mRNA expression levels of LC3, p62, PINK1 and Parkin between the control group and aging group. B and C The protein expression levels of LC3, p62, PINK1 and Parkin between the control group and aging group. D Immunofluorescence staining was used to detect the localization and expression of LC3 in the ovarian tissues of the control group and aging group. Scale: 200 μm, 100 μm, 50 μm. E The mRNA expression levels of Bcl2 and Bax between the control group and aging group. B and C The protein expression levels of Bcl2 and Bax between the control group and aging group. Error bars, mean ± SEM. n = 6–8 per group. *P < 0.05, **P < 0.01