Fig. 3

HAT1 inhibition induces meiotic defects and decreases oocyte quality. (A) Morphology of spindles and chromosomes in oocytes from the CTL (n = 30) and AA (n = 46) groups. Scale bar: 25 μm. (B) Statistical analysis of meiotic defects rate. The data are shown with three independent experiments. (C) Morphology of spread chromosomes in oocytes from the CTL (n = 27) and AA (n = 31) groups. Chromosomes were stained with DAPI (blue). (D) Statistical analysis of aneuploidy. The data are shown with three independent experiments. (E) Representative images of 2 cell embryo and blastocyst embryo in the CTL (n = 83 MII oocytes) and AA (n = 88 MII oocytes) groups. Scale bar: 100 μm. (F)-(G) Percentage of 2 cell embryos and blastocysts. The data are shown with at least three independent experiments. Data are presented as mean ± SEM, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test