Fig. 5

Association between increases in ER stress and exosomal expression levels in the caput epididymis of obese mice. A-B Representative Western blot analyses of candidate proteins PDIA3, RRBP1 in the caput epididymis of control mice and obese mice, respectively. C-D Representative Western blot analysis of candidate protein CRELD2 and exosome marker CD63 in the caput epididymis of control mice and obese mice, respectively. E-F The caput epididymis of the obese mice showed high level of ER stress with the up-regulated expression of IRE1α and PERK. G-H Representative Western blot analysis of exosome marker CD63 in the exosomes of control mice and obese mice, respectively. The relative quantification of the tissue proteins was normalized to β-actin and the exosome proteins were normalized to TSG101. All data were presented as mean ± SD, *P < 0.05