Fig. 4

Validation of the RNA sequencing results using RT-qPCR. A Bars extending to the right or left of zero coordinate represent up or down regulated genes, respectively. Black bars represent expression of transcript in RNA sequencing while grey bars represent expression of the same transcript presented by RT-qPCR. B Comparison of expression of 7 selected genes between the two groups. Asterisk (*) above the bar represents statistical difference in RT-qPCR experiments. *P < 0.05; ***P < 0.001